1-Methylpseudouridine is a substitute for uridine in modified mRNA. This substitution has shown to increase transfection by reducing immunogenicity. The 1-methylpseudouridine substituted mRNA increases protein expression due to low immune. 1-Methylpseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density.

[1] Yuri V Svitkin, et al. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density. Nucleic Acids Res. 2017 Jun 2;45(10):6023-6036.

[2] Sahin U., Kariko K., Tureci O.. mRNA-based therapeutics–developing a new class of drugs. Nat. Rev. Drug Discov. 2014; 13:759–780.